Joint Development of Mutant Mice with Asian-Pacific Researchers


As a part of our mission, we actively collaborate with investigators in academia in the Asia-Pacific region to develop genetically engineered mice (GEM) as a resource for life science research. In this collaborative mission, LARGE at CLST/CDB will generate GEM, and the role of the collaborating investigators is to propose genes of interest and to characterize phenotypes. It is requested that collaborating investigators cover a part of costs involved in the process:
1) GEM by homologous recombination in ES cells (KO/KI): 1,500,000 yen
LARGE will generate KO/KI mice starting from construction of targeting vectors. There is an extra charge of 400,000 yen if the request is to generate KO/KI mice from a C57BL/6N (inbred) background ES cell line. LARGE recommends the resulting KO/KI mice to be shipped as frozen embryos to avoid a risk of microbiological contaminations and to minimize complications in live animal shipment. However, if the collaborating investigator does not have technical support of handling cryopreserved embryos for rederivation, LARGE can accommodate preparation and shipment of F1 heterozygotes as live mice with an extra charge. We regularly arrange international shipments of cryopreserved embryos or live mice with World Courier <>. It is requested that the investigator covers shipping cost.
2) Transgenic (TG) mice by microinjection into zygotes: 200,000 yen
Typically two hundred frozen zygotes injected with DNA will be sent to the collaborator. This requires the investigator to arrange technical support to rederive mice from frozen embryos. If such arrangements are not possible at investigator’s hands, depending on the space availability, LARGE may propose possible compromises and will try to accommodate specific needs upon discussion with the investigator.

In addition:
1) The investigator must agree and arrange to receive the embryos/mice that are prepared under the CDB's microbiological standards (
2) The investigator must agree to include designated members of LARGE as coauthors in the first relevant publication describing the generation and characterization of the GEM resulting from the collaboration. LARGE must be given an opportunity to review the draft manuscript for consent before its submission and publication.
3) In any publications reporting the use of the GEM, they must be described with the Accession Number (Acc. No.) assigned by LARGE and its website (KO/KI,;TG,
4) After publication of the first relevant paper about the GEM, the strain will be deposited to RIKEN BioResource Center (BRC). The mice will be distributed to researchers around the world upon requests under the conditions that the collaborating investigator can define in part. Third parties receiving the requested GEM will also be asked to cite the first relevant publication, and to describe the GEM with the assigned Acc. No. and the website (KO/KI,;TG,

If you are interested in the joint development of mutant mice, please contact us: mutant(at)

理化学研究所 ライフサイエンス技術基盤研究センター 生体モデル開発ユニット/生体工学研究チーム
〒650-0047 神戸市中央区港島南町2-2-3