Services Offered

Genome Sequencing
Genome analysis using next-generation high-throughput sequencers

Advantages

  • Various types of genome analysis such as whole-genome resequencing, targeted resequencing (mutation analysis), de novo analysis and metagenomic analysis are available.
  • The type of sequencer used for analysis can be selected according to the purpose of the analysis and the customer’s budget.
  • We can meet a range of needs with the various genome analysis techniques we have on offer. This includes whole-genome analysis and single-nucleotide polymorphism (SNP) analysis for a variety of species from viruses and bacteria to mammals. Please feel free to discuss your needs with us.

Services

High-throughput Sequencing Analysis with Illumina HiSeq2500

Features of Sequencers
Platform HiSeq2500 High Output Mode HiSeq2500 Rapid Run Mode
Manufacturer illumina illumina
Amplification Method for DNA Templates Bridge PCR Bridge PCR
Sequencing Method Sequencing by synthesis Sequencing by synthesis
Read Length 50 or 100 bp 50/100/150/250 bp
Data Output per Run 540-600 Gb
(2 x 100 bp)
250-300 Gb
(2 x 250 bp)
Advantages A large volume of data is generated.
Paired-End sequencing possible
Multiplexing possible
A large volume of data is generated in a couple of days.
250bp PE sequencing possible

* Figures based on catalogue specifications. Current as of March 2016.

Exome Sequencing

Using a commercially available kit to enrich the exon regions, it is possible to obtain deep coverage of exon regions in comparison to sequencing the entire genome.
Hence, this method is suitable for conducting highly effective mutation analysis which focuses on specific exon regions.
When used in combination with multiplexing techniques, it is possible to analyze numerous samples for the same cost.

←An example of exome sequencing analysis using CLC Genomics Workbench
Deep coverage of the exon regions is obtained (as indicated in the boxes with dashed borders).

Delivered data

  • Sequencing data (Genome sequences and quality information after basecalling)
  • Contiguous sequence data (For genome sequencing or sequence assembly)
  • Genome mapping data (When a reference genome exists)
  • SNP Report (For SNP analysis)
  • Please contact us to discuss issues such as other file formats and analysis tools.

Bioinformatics analysis

An example of SNP analysis using CLC Genomics Workbench.
A report on the SNP type, location, frequency and read coverage is generated.

Depending on your needs, you can choose between the genome assembly software provided with the sequencer platform or CLC Genomics Workbench.
Hybrid assembly, using sequence data generated by different sequencers each with different read lengths, is also available.
(When using CLC Genomic Workbench)

SNP Analysis (CLC Genomics Workbench)
SNPs are detected and reported. If an ORF or CDS annotation of the reference sequence exists, amino acid sequence changes resulting from SNPs will also be detected and reported.
Detection and reporting of small insertion/deletion
polymorphisms (DIP) are also supported.