List of Services
We offer the CAGE technique to ovesrseas as well as domestically. The others are available only in Japan.
They rely on next-generation sequencers:
CAGE is a unique technique developed by RIKEN. It extracts and sequences short tags from the 5’ ends of capped messenger RNA molecules. Using the next-generation sequencers, this technique makes it possible to analyze genome-wide gene expression. CAGE provides a wider dynamic range and a more quantitative gene expression profile than conventional microarray analysis. It is possible to obtain information about transcription start sites specific to a sample (from a specific tissue, cell or stage, etc).
Genome Sequencing >
- Whole-genome Sequencing
- By sequencing the whole genome, mutation analysis can be conducted.
With a next-generation sequencer, for example Hiseq2500, it is possible to output over 100 times the data of the human genome in a single sequencing run.
- De novo Sequencing
- The genome sequence of an organism without reference can be determined through whole-genome sequencing.
- Targeted Resequencing
- It is possible to analyze mutation types and locations by making intensive analysis for the exon regions or specific genome regions of interest.
Chromatin Immunoprecipitation sequencing (ChIP-Seq) allows for the identification of genome sites subject to histone modification or DNA-protein interaction sites such as transcription factors by analyzing the base sequence of DNA fragments isolated through chromatin immunoprecipitation. The techniques are also applicable to the detection of modified DNA with methods such as MBD-Seq.
- Directional RNA-Seq
- Non-directional RNA-Seq
- Quantitative expression analysis and structure investigation for transcripted products are conducted through investigating the sequence of RNA with next-generation sequencers. This technique also makes it possible to detect the sequence of novel transcripted products and splice variants.
Additionally, we offer directional RNA-Seq analysis through which it is possible to detect the direction of the strand of RNA in the cell. This allows for the detection of anti-sense RNA which is difficult to detect with non-directional RNA-Seq.
Small RNA-Seq >
The large volume of data generated by next-generation sequencers allows for the analysis of small RNA such as microRNA (miRNA) and small interfering RNA (siRNA), which may be involved in the regulation of transcription and that of gene expression after transcription. Compared to microarray and qRT-PCR (quantitative PCR) analysis, the small RNA-Seq offers a more exhaustive analysis (including detection of novel small RNA), and it makes it possible to analyze the expression frequency of transcripts quantitatively regardless of the degree of expression.