Zygote Electroporation

CRISPR/Cas9を用いたZygote electroporationによる遺伝子改変マウスの作製


1. Hashimoto, M., and Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing.

2. Sci Rep 5, 11315.Hashimoto, M., Yamashita, Y., and Takemoto, T. (2016). Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Dev Biol 418, 1-9.