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Zygote Electroporation
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CRISPR/Cas9を用いたZygote electroporationによる遺伝子改変マウスの作製
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References
1. Hashimoto, M., and Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing.
2. Sci Rep 5, 11315.Hashimoto, M., Yamashita, Y., and Takemoto, T. (2016). Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Dev Biol 418, 1-9. |
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