Knockinマウスの作製

Single strand DNA(ssDNA) donor: donorにssDNAを用いる方法

Reference: Quadros, R.M., et al. (2017). Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol 18, 92.

PITCh(Precise Integration into target Chromosome) system: MMEJを利用したPITCh systemによる方法

References:

Abe, T., et al. (2020). Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes. Cell Reports 31, 107653

Sakuma, T., et al. (2015). MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 2016 Jan;11(1):118-33.

Homology recombinationによる方法

References:

Abe, T., et al. (2020). Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes. Cell Reports 31, 107653