Reporter Mice for Live Imaging

LARGE has recently developed a number of fluorescent reporter mouse lines useful for live imaging of embryos and tissues to aid better understanding of dynamic developmental processes. The following list of reporter mice are available to the scientific community. Please contact "mutant(at)cdb.riken.jp" to request the reporter mouse line of your interest.

Download Excel File

contact

Mouse Request: mutant(at).cdb.riken.jp

Applications

Line names

CDB Acc. No.

Ref.

Cell organelles

Nucleus

R26R-H2B-EGFP

CDB0203K

1

R26-H2B-EGFP

CDB0238K

1

R26R-H2B-mCherry

CDB0204K

1

R26-H2B-mCherry

CDB0239K

1

R26R-H2B-mKeima-Red

CDB0205K

1

R26-H2B-mKeima-Red

CDB0240K

1

Membrane

R26R-Display-Kikume Green Red

CDB0207K

1

R26-Display-Kikume Green Red

CDB0242K

1

R26R-Display-Venus

CDB0208K

1

R26-Display-Venus

CDB0243K

1

R26R-Display-mCherry

CDB0209K

1

R26-Display-mCherry

CDB0244K

1

R26R-Lyn-Venus

CDB0219K

1

R26-Lyn-Venus

CDB0254K

1

Nucleus & Membrane

R26R-RG

CDB0227K

2

R26-RG

CDB0237K

2

Golgi

R26R-Golgi-EGFP

CDB0211K

1

R26-Golgi-EGFP

CDB0246K

1

R26R-Golgi-mCherry

CDB0212K

1

R26-Golgi-mCherry

CDB0247K

1

Mitochondria

R26R-Mito-EGFP

CDB0216K

1

R26-Mito-EGFP

CDB0251K

1

Cytosol

CAG-KikGR-1

CDB0201T-1

4

CAG-KikGR-2

CDB0201T-2

4

 

Microtubule

R26R-EGFP-Tuba

CDB0210K

1

R26-EGFP-Tuba

CDB0245K

1

R26R-EB1-EGFP

CDB0213K

1

R26-EB1-EGFP

CDB0248K

1

R26R-hEMTB-EGFP

CDB0214K

1

R26-hEMTB-EGFP

CDB0249K

1

Actin

R26R-Venus-Moesin

CDB0217K

1

R26-Venus-Moesin

CDB0252K

1

R26R-Venus-Actin

CDB0218K

1

R26-Venus-Actin

CDB0253K

1

 

Focal contacts

R26R-EGFP-Paxillin

CDB0221K

1

R26-EGFP-Paxillin

CDB0256K

1

 

Tight junction

R26R-ZO1-EGFP

CDB0225K

7

R26-ZO1-EGFP

CDB0260K

7

 

Adherence junction

R26R-PHA7-EGFP

CDB0226K

9

R26-PHA7-EGFP

CDB0261K

9

Cell cycle

Fucci2 (G1)

R26R-mCherry-hCdt1(30/120)

CDB0229K

3

R26-mCherry-hCdt1(30/120)

CDB0264K

3

Fucci2 (S/G2/M)

R26R-mVenus-hGem(1/110)

CDB0230K

3

R26-mVenus-hGem(1/110)

CDB0265K

3

Fucci2 (G1 & S/G2/M)

R26p-Fucci2

CDB0203T

3

 

Wnt signaling pathway

R26-WntVis

CDB0303K

8

Publications

1) Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging.
Abe T, Kiyonari H, Shioi G, Inoue K, Nakao K, Aizawa S, Fujimori T.
Genesis. 2011 Jul;49(7):579-90. doi: 10.1002/dvg.20753. Epub 2011 Jun 17.
PMID: 21445964


2) A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
Shioi G, Kiyonari H, Abe T, Nakao K, Fujimori T, Jang CW, Huang CC, Akiyama H, Behringer RR, Aizawa S.
Genesis. 2011 Jul;49(7):570-8. doi: 10.1002/dvg.20758. Epub 2011 Jun 17.
PMID: 21504045


3) Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter.
Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.
Development. 2013 Jan 1;140(1):237-46. doi: 10.1242/dev.084111. Epub 2012 Nov 22.
PMID: 23175634

4) Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape.
Kurotaki Y1, Hatta K, Nakao K, Nabeshima Y, Fujimori T.
Science. 2007 May 4;316(5825):719-23. Epub 2007 Apr 19.
PMID: 17446354


5) Reporter mouse lines for fluorescence imaging.
Abe T, Fujimori T.
Dev Growth Differ. 2013 May;55(4):390-405. doi: 10.1111/dgd.12062. Epub 2013 Apr 29. Review.
PMID: 23621623


6) Live imaging of early mouse embryos using fluorescently labeled transgenic mice.
Abe T, Aizawa S, Fujimori T.
Methods Mol Biol. 2013;1052:101-8. doi: 10.1007/7651_2013_20.
PMID: 23674079


7) Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.
Katsunuma S, Honda H, Shinoda T, Ishimoto Y, Miyata T, Kiyonari H, Abe T, Nibu K, Takai Y, Togashi H.
J Cell Biol. 2016 Feb 29;212(5):561-75. doi: 10.1083/jcb.201509020.
PMID: 26929452


8) R26-WntVis reporter mice showing graded response to Wnt signal levels.
Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H.
Genes Cells. 2016 Jun;21(6):661-9. doi: 10.1111/gtc.12364. Epub 2016 Mar 31.
PMID: 27030109

9) Apical constriction in distal visceral endoderm cells initiates global, collective cell rearrangement in embryonic visceral endoderm to form anterior visceral endoderm.
Shioi, G., Hoshino, H., Abe, T., Kiyonari, H., Nakao, K., Meng, W., Furuta, Y., Fujimori, T., and Aizawa, S. (2017). Developmental biology.